The most used markers for individual identification and analysis of paternity are microsatellite loci. PCR amplification of the 9 microsatellite loci was carried for 216 broodfishes already present and 188 candidate broofishes, for then visualize the product of the amplification through capillary electrophoresis. Genotyper 3.7 was used to characterize a microsatellite profile for every Finally, the program ML Relate allowed to attribute a value of relatedness for all the pair wise comparisons among the 188 originating reproducing candidates from Valle…